Anna Kathryn Holbrook, born Anna Kathryn York on April 18, 1957, in Fairbanks, Alaska, is an American television actress best known for her role as Sharlene Frame Hudson on the NBC daytime soap opera Another World.

She received a Daytime Emmy Award for Outstanding Supporting Actress in a Drama Series in 1996 for her portrayal of Sharlene, a character who suffered from multiple personality disorder and was a victim of sexual abuse. She grew up in Tucson, Arizona, after her father, Don Joseph York, was killed in South Vietnam during the Vietnam War.

Anna Kathryn Holbrook began her career appearing in television series such as Hart to Hart and Dallas. In 1988, she moved to New York City to perform on off-Broadway stages before being cast in Another World.

Although soap operas were not initially part of her plans, her agent's advice changed her mind, and she stayed with the show until 1991, returning briefly in later years.

She has also appeared in numerous guest-starring roles on shows like Law & Order, Law & Order: Special Victims Unit, Spin City, The West Wing, Bull, Unbreakable Kimmy Schmidt, Blue Bloods, Homeland, Hunters, The Blacklist, Harlem, and The Equalizer.

She appeared in the 1994 comedy film I Love Trouble and had a recurring role as Dr. Hannah Young on the ABC daytime soap opera One Life to Live in 2007.

In 2021, she played Debbie in the Sister Swap film franchise. She starred as the mother of real-life sisters Kimberly Williams-Paisley and Ashley Williams, who are also the real-life daughters of Holbrook's best friend.

Anna Kathryn Holbrook has been married to Bruce Holbrook since 1979, and they have two children.